11/7/2022 0 Comments Cytoscape viral![]() The RNAi mechanism could inhibit different types of virus infections such as rotavirus, influenza virus, and HIV-1. Similarly, during viral infection, the dsRNA of the viruse is cleaved by Dicer to generate siRNAs, which finally target the complementary viral RNA. One of the siRNA strands bound the RISC complex RISC and cleaves the target mRNA. Identification of exogenous dsRNAs through PRRs results in the generation of siRNAs (21-25nt in length) mediated by Dicer. Using a guide RNA strand, the RISC complex could eighter suppress the mRNA translation or degrade the mRNA by Ago slicer activity. The most critical component of these complexes is argonaute RISC catalytic component (AGO) proteins, Ago2 in particular. Initially, endogenous or exogenous dsRNAs bounds to form siRNA or miRNA-induced silencing complex (RISC). miRNA biogenesis involves Drosha, Dicer and RNAase III proteins, while siRNA processing is exclusively associated with Dicer function. The RNAi system as an innate immunity pathway is mainly mediated by two non-coding molecules, including microRNA (miRNA) and small interfering RNA (siRNA). ![]() RNAi is a mechanism of post-transcriptional gene silencing in eukaryote and human cells, that inhibit virus replication and expression of various viral proteins. Host cell pattern recognition receptors (PRRs) could distinguish viral dsRNA as a foreign pathogen and respond to them by activating several antiviral pathways such as the RNA interference (RNAi) mechanism that is critical for early defense against viral invasion. Coronavirus is capable of producing double-stranded RNA (dsRNA) during the infection cycles. The respiratory system and lungs are most susceptible to damages resulting from the virus that leads to failed functions of the respiratory system, severe acute pulmonary disorders, and consequent mortality. After the Chinese epidemic peak, other countries, especially South Korea, Italy, and Iran saw a significant spread of the disease. SARS‐CoV‐2 categorized in beta-coronaviruses lineage and is closely related to the SARS‐CoV virus. The virus was first identified in Wuhan, Hubei, China, in 2019 and spread worldwide. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus responsible for severe respiratory disease (COVID-19). Ultimately, the RNA interference mechanism as a crucial antiviral defense system was suggested to be dysregulated in COVID-19 patients. Our findings demonstrated that the expression of Dicer, Drosha, and Ago2 was statistically altered in COVID-19 patients compared to healthy subjects. This study aimed to assess the expression level of the mentioned mRNAs in COVID-19patients compared to healthy individuals. Dicer, Drosha, Ago2, and DGCR8 are essential components of the RNAi system, which is supposed to be dysregulated in COVID-19 patients. ![]() RNA interference (RNAi) is a mechanism of post-transcriptional gene silencing that plays a crucial role in innate viral defense mechanisms by inhibiting the virus replication as well as expression of various viral proteins. This virus was initially identified in Wuhan city, a populated area of the Hubei province in China, and still remains one of the major global health challenges. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the novel coronavirus causing severe respiratory illness (COVID-19). ![]()
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